Not sure if it's still on my clothes

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I took the cream (Lyclear Dermal) yesterday and saw how the mites go, sealed most of my clothes including bed and sofa and washed in high temperature whatever I need for the meantime. I keep feeling that the itching increasing around 20 minutes after I wear some of my clothes, or sit on my sofa cover that I washed as well. If the fabric still infected should the reaction be so fast? Is covering the skin reduces the itches ? Also, do the itches increase during the night also when the mites are dead?

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