ANA Direct - positive abnormal?

Posted , 6 users are following.

This discussion has been locked due to a period of inactivity. Start a new discussion

I had the ANA DIRECT test done and it came back positive abnormal.  What does this mean?  How high, makes a positive and what does the abnormal mean?  Thank you!  Any hekp would be very much appreciated..

1 like, 22 replies

Report

22 Replies

Next
  • Posted

    My ANA is positive as well, at 1:640 dilution. The higher the dilution you're positive at the more likely that means you have an autoimmune process going on. Many people with no disease are positive at low dilutions. A lot of people with Hashimotos have positive ANA, so unless you have symptoms of other autoimmune conditions, or other positive autoantibodies, it could likely be due to the Hashimotos. They also look at the pattern of staining. I'm not totally familiar with it but I think speckled patterns could be many things, while other staining patterns are more associated with lupus.

    Report
    • Posted

      Hi Jennifer/Melissa

      My ana ws 1 in 80 Is this high? I am unable to find the original report to ett ref range but BH did the test.

      ​TIA. I am hopeless at maths.

      Report
    • Posted

      1:80 is considered low. The higher the dilution it's positive, the more likely it means something. Like I'm positive at 1:640, meaning my sample was diluted a lot and still came up positive! Hope that helps!

      Report
  • Posted

    melissa

    Who did the test Was it BH or NHS?

    Report
    • Posted

      I had the ANA DIRECT done through LAB Corp.  All it said was Positive Abnormal.  There was no titer or staining. Just positive abnormal. That was in January of 2016, the I just had another ANA done, that was called ANA Elisa, a week ago, but it came up negative.  That test was done by my local hospital.  I was wondering why one would be positive and then on a different one be negaitive.  Now at the time that it was positive, I felt awful for about 10 months with low grade fevers, swollen lymph nodes, severe fautigue and I just didn't feel good.  This newer Ana elisa, when I got this one, I felt fine except for swollen and stiff fingers for 1 to 2 hours each morning and nausea after every meal.  My doctor did it at the time to look for RA.  He said I might be in some kind of flare up.  Now the first ANA Direct I got (when I was sick for 10 months) I got because I was worried about Lupus, which my mom and aunt have.  But I never went further then the ana, because I started to feel better, go figure, when I was just starting to get somewhere..lol.  I didn't go any farther with it because I read that if you are not in a flare up, that your test can up negaitive.. not sure if this is true or not?  I did finally find something on the net about that ANA DIRECT test though.  I read that they do it by the Athena Bead staining.  I guess they test the ana through about 9 antigens.  I guess the most relevent ones.  And if any of those antigents at all are over 100 of them, then that makes it positive.  I also found out that that anything under 100 is considered negative, 100 to 120 is equivelent, and anything over 120 is positive.  So I guess my number had to be over 120.  But they don't tell you what antigen that was high or how many of them  ex.. of antigens.. Anti-dsDNA, Anti-La/SS-B, Anti-Ro/SS-A, Anti-Sm, but 9 of them I believe.  Not sure which ones though. Also, the ANA direct is more accurate then other ANA test.  I also found out that if you get a positive ANA Direct that you should immediatly get the ana IFA done.  That would help with identifing which antibody was high, But I didn't at the time, because I didn't know.  ...lol... I guess I'm answering my own question.  I did ALOT of reading yesterday.  But I am still confused about why this ANA elisa is negative?  Can it become negative if I'm not in a flare up after being positive?    Thanks for listining and your reply.  I wanted to write what I found out about the ANA DIRECT because it seems that all over the internet, everything I could find, no one knew anything about it, but wanted to know more about it. 

      Report
    • Posted

      Interesting, I didn't know about ANA direct. I had all my autoantibodies tested separately after I got the positive ANA titer/staining, so was able to see all the results (all negative fortunately). You're right that the autoantibodies are more informative, since the ANA titer is pretty nonspecific. Could be different autoimmune diseases or nothing at all. I think it is possible that levels fluctuate with flares since I remember reading that certain things are treated to monitor the disease, but I'm not sure specifically. Good luck with this all rolleyes

      Report
    • Posted

      Yes.. some places I read that the ana could be negative if not in a flare, and other things I read say no, that once positive, always positive.  Also, I'm wondering if the one test was a false positive or the other a false negative..lol.  I guess I won't be able to.  I'm sure that I will just have to wait until I feel bad again and redo the Ana test.  Next time, I'm going to the ana ifa with the reflex though..I think it will give more info then just a positive or negative..  Boy.. this ANA stuff is really confusing..lol.  Best wishes to you also Jennifer!

      Report
    • Posted

      Yes.. I'm in the U.S. It was my family doctor that ordered the blood work.  At the Monroe Noxen health center.

      Report
    • Posted

      In the UK the medical profession don't bother as much. I paid to go privately and the Company had a special deal on offer for antibody testing. The extra cost was only marginal so I decided to go ahead . Nearly fainted when the antibodies came back positive.

      Report
    • Posted

      Yes.. I might pay privately to get the antibodies tested.  It's so expensive though.  But I guess it would  be worth it if something came back positive.  Lupus is a big one in my family.  My mom and aunt have it. 

      Report
    • Posted

      Yes.. US.  To get all the antibodies tested here, it's about $700.00 dollars.  I think I'll go for it though.  Your right.  It's so important.

      Report

Join this discussion or start a new one?

New discussion

Report as inappropriate

Thanks for your help!

We want the forums to be a useful resource for our users but it is important to remember that the forums are not moderated or reviewed by doctors and so you should not rely on opinions or advice given by other users in respect of any healthcare matters. Always speak to your doctor before acting and in cases of emergency seek appropriate medical assistance immediately. Use of the forums is subject to our Terms of Use and Privacy Policy and steps will be taken to remove posts identified as being in breach of those terms.

newnav-down newnav-up